ICAN Omics metabolomics
Goals
The strategy developed within our platform is based on the profiling of a large number of metabolites using multidisciplinary skills combining bioinformatics, statistics tools and biochemical databases for the interpretation of results using a high-resolution mass spectrometer coupled with chromatographic systems.
Services and Equipment
- Metabolome study
- Acquisition and interpretation of metabolomic profiles
- Analysis and quantification of several families of metabolites
- Advice and implementation of metabolomic assays
- Annotation, identification and structural elucidation of metabolites (HRMS, MS/MS)
- Development and realization of specific methods (‘Custom method’)
Targeted approach
Absolute quantification of amino acids, tryptophan pathway, choline metabolism pathway (TMAO, Choline…), short chain fatty acids (SCFAs), bile acids (in dvlpt)
Profiling method
Detection and relative quantification of compounds related to the microbiota and belonging to the family:
- Method 1: amino acids, organic compounds, phenols, methylamines and some indoles
- Method 2: Acylcarnitines, free fatty acids and bile acids
Untargeted approach
Study of the global metabolome through several chromatographic methods.
Detection of more than 200 metabolites in biofluids (plasma-serum)
Biological study matrices.
Serum-Plasma, CSF, urine, faeces, Cecum, PBMC and glial cells.
Other matrices under pilot study conditions
(Contact the operational manager for more information – Farid Ichou f.ichou@ihuican.org )
Networks and Collaborations
- Training: reference platform for the Biomics course of the STA2E master’s degree from Université Paris Est Créteil
- Local networks: UMS RésOmique (Sorbonne University), AP-HP, Inserm U1166
- National networks: MetaboHub, RFMF, INSERM Microbiota Consortium
- International networks: Metabomeeting, european regional metabolomics networks, Metabolomics Society
Access conditions
Access to our platform is via an analytical request. Download the form here
You can view the terms of use here.
The procedure after receipt of the “request form” is as follows:
- Submission of the request form to the platform via “ Analytical request ” to the platform manager Farid Ichou: f.ichou@ihuican.org
- Project evaluation and establishment of a tariff proposal
- Validation of the specifications and signature of an estimate/agreement
- Receipt of samples
- Execution of the works according to the specifications (or the estimate)
- Presentation of the final study report
Certificates
IBiSA label obtained in 2020 ( https://www.ibisa.net/plateformes/icanalytics-635.html )
Team and contact
Philippe Lesnik, (DR, UMR ICAN 1166)
Scientific Director
Maharajah Ponnaiah (IR, ICAN)
Biostatistician
Sora Lecocq (Tech, ICAN)
Technician
Scientific Committee
Prof. Fanny Mochel (ICM)
dr. Foudil Lamari (APHP)
dr. Ivo Gomperts Boneca (Pasteur Institute)
dr. Francois Fenaille (CEA)
Wilfried Le Goff UMR (ICAN 1166)